Wide Spread of Plasmid-Mediated Antibiotic Resistant Bacteria in Imported Fishery Products

Food contamination with extended-spectrum beta-lactamase (ESBL)-producing bacteria is recognized as one of the public health concerns worldwide. Although fish and fishery products at retail in Japan have relied on imported materials, no apparent evidences exist prevalence property genetic transfer ESBL-producing those food specimens. This study aimed to investigate plasmid-mediated antibiotic-resistant products. Fifteen retailed Japan, from Southeast South Asia during January March 2020, were examined detect AmpC/ESBL-producing Escherichia coli, or Vibrio spp. through culture buffered peptone water (BPW) alkaline (APW), followed by selective enrichment CHROMagar ECC containing cefotaxime/meropenem. A total 172 strains finally isolated 15 samples. 16S rRNA sequencing gene revealed predominant distribution Stenotrophomonas maltophilia E. coli ECC, Enterococcus Vibrio, respectively. Amongst, Klebsiella pneumoniae, V. vulnificus, parahaemolyticus ESBL-producers harboured CTX-M-15, 55, 27. Aeromonas caviae AmpC β-lactamase-producers CMY-8 CMY-2, One Enterobacter cloacae complex shrimp sample was resistant meropenem NDM-1. assay that all ESBLs, except carbapenemase-producing spp., transferred CTX-M NDM-1 ATCC25922 BL21. Overall, our data indicated frequent materials AmpC/ESBL bacteria, possible transferability genes intestinal humans.